15522676233
15522676233
技術文章
更新時間：2020-03-10
點擊次數：2093
Cat No.: BLPSN-0981P
| Tag | mFc |
| Host Species | 2019-nCoV |
| Background | 2019 Novel Coronavirus (2019-nCoV) is a virus (more specifically, a coronavirus) identified as the cause of an outbreak of respiratory illness first detected in Wuhan, China. |
| Description | A DNA sequence encoding the receptor binding domain (RBD) of 2019-nCoV spike was expressed in human cells and fused with the Fc region of mouse IgG1 at the C-terminus. |
| Source | Human Cells |
| Predicted N Terminal | Arg |
| Molecular Weight | The recombinant receptor binding domain (RBD) of 2019-nCoV spike consists of 457 amino acids and predicts a molecular weight of 51.5 kDa. |
| Purity | > 95% as determined by SDS-PAGE. |
| Endotoxin | <1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (RBD, mFc Tag)(BLPSN-0981P),the EC50 of 2019-nCoV Spike Protein (RBD, mFc Tag) is 15-50ng/mL. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The 2019-nCoV Spike Protein is stable for up to 1 year from date of receipt at -20℃ to -80℃. |
| Usage | For Research Use Only |
| Storage | Store the 2019-nCoV Spike Protein under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
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